1463 Keratinocyte autophagy regulates wound healing through MCP-1-mediated keratinocyte migration and fibroblast activation

Wound re-epithelialization: modulating keratinocyte migration in wound healing.

An essential feature of a healed wound is the restoration of an intact epidermal barrier through wound epithelialization, also known as re-epithelialization. The directed migration of keratinocytes is critical to wound epithelialization and defects in this function are associated with the clinical phenotype of chronic non-healing wounds. A complex balance of signaling factors and surface protei...

Erk5 controls Slug expression and keratinocyte activation during wound healing.

Reepithelialization during cutaneous wound healing involves numerous signals that result in basal keratinocyte activation, spreading, and migration, all linked to a loosening of cell-cell adhesion structures. The transcription factor Slug is required for this process, and EGF treatment of human keratinocytes induced activating phosphorylation of Erk5 that coincides with slug transcription. Acco...

Signaling Pathway Proliferation and Migration via the ERK Healing by Promoting Keratinocyte Adiponectin Regulates Cutaneous Wound

Activation of keratinocyte fibronectin receptor function during cutaneous wound healing.

Keratinocytes freshly isolated from unwounded skin could not attach and spread on fibronectin (FN)-coated culture dishes and could not bind and phagocytose FN-coated beads. These adhesive functions were activated, however, in keratinocytes that were isolated from healing wounds. Moreover, adhesiveness of basal keratinocytes to FN substrata was activated during epidermal cell or explant culture....

O-8: Clinical Application and Evaluation of Autologous Keratinocyte and Fibroblast Cells Culture on Horse Open Wound Healing

KFGS (Keratinocyte fibrin glue suspension) and FKFGS (Fibroblast keratinocyte fibrin glue suspension) methods were performed in this study. Materials and Methods: Four healthy adult horses were used in this study (350 ± 117). Fibroblast cells have been separated by explant culture method from neck, keratinocyte cells have been separated by enzyme digestion from lib skin samples and Fibrinogen h...